Search results for "Combinatorial peptide ligand library"

showing 3 items of 3 documents

Orange proteomic fingerprinting: From fruit to commercial juices.

2015

Combinatorial peptide ligand library technology, coupled to mass spectrometry, has been applied to extensively map the proteome of orange pulp and peel and, via this fingerprinting, to detect its presence in commercial orange juices and drinks. The native and denaturing extraction protocols have captured 1109 orange proteins, as identified by LC-MS/MS. This proteomic map has been searched in an orange concentrate, from a Spanish juice manufacturer, as well as in commercial orange juices and soft drinks. The presence of numerous orange proteins in commercial juices has demonstrated the genuineness of these products, prepared by using orange fruits as original ingredients. However, the low nu…

0301 basic medicineProteomicsProteomeOrange (colour)01 natural sciencesAnalytical ChemistryBeverages03 medical and health sciencesTandem Mass SpectrometryLc ms msFood scienceOrange juiceLC-MS/MSPeptide ligandOrange fruitPlant ProteinsOrange juiceCombinatorial peptide ligand library; LC-MS/MS; Orange fruit; Orange juice; Protein; Proteomics; Food Science; Analytical ChemistryChromatographyChemistryProtein010401 analytical chemistryGeneral Medicine0104 chemical sciences030104 developmental biologyFruitProteomeCombinatorial peptide ligand libraryCitrus × sinensisFood ScienceCitrus sinensisFood chemistry
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Proteomic fingerprinting of mistletoe (Viscum album L.) via combinatorial peptide ligand libraries and mass spectrometry analysis

2017

Abstract Combinatorial peptide ligand libraries (CPLLs), coupled to mass spectrometry (MS) analysis, have been used to investigate in depth the proteome of Viscum album L. (VA), commonly named European mistletoe, in order to provide a first proteomic fingerprinting. For this purpose, the proteins were captured via CPLLs at two different pH values (acidic and neutral). A total of 648 non-redundant proteins were identified by using two different databases. The two pH values, chosen for bead incubations, have contributed to increment the capture ability: 56% and 31% of CPLLs species were respectively recognized at pH 7.2 and at pH 2.2. Finally the biological function of identified proteins was…

0301 basic medicineProteomicsProteomeViscum albumCancer therapyBiophysicsComputational biologyBioinformaticsProteomicsMass spectrometryBiochemistry03 medical and health sciencesHuman health0302 clinical medicinePeptide LibraryViscum albumPeptide libraryPeptide ligandPlant ProteinsbiologyMass spectrometryPlant Extractsbiology.organism_classificationEuropean mistletoeProteomic fingerprinting030104 developmental biology030220 oncology & carcinogenesisProteomeCombinatorial peptide ligand library; European mistletoe; Mass spectrometry; Proteomic fingerprinting; Plant Extracts; Plant Proteins; Proteome; Viscum album; Peptide Library; Proteomics; Biophysics; BiochemistryCombinatorial peptide ligand library
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Proteomic fingerprinting of apple fruit, juice, and cider via combinatorial peptide ligand libraries and MS analysis

2018

Combinatorial peptide ligand libraries coupled to MS was applied to extensively map the proteome of apple fruit, and to detect its presence in commercial apple juice and cider to evaluate their authenticity and genuineness. Using the Uniprot_Malus database, 96 proteins were detected in apples, among which 30 proteins were specifically captured via combinatorial peptide ligand libraries. Next, three proteins, previously recognized in fruits, were found in apple juice, which were involved in cellular metabolism of fruit maturation and in allergenic reactions. On the other hand, only one Malus allergen was identified in cider beads eluate, demonstrating that the industrial processes did not pr…

ProteomicsMalusProteomeClinical Biochemistry02 engineering and technology01 natural sciencesBiochemistryMass SpectrometryAnalytical ChemistryFruit maturationPeptide LibraryApple allergensPeptide ligandPlant ProteinsApple allergens; Apple fruit juice and cider; Combinatorial peptide ligand library; Mass spectrometry; Proteomic fingerprintingCellular metabolismbiologyChemistry010401 analytical chemistryMs analysis021001 nanoscience & nanotechnologybiology.organism_classificationProteomic fingerprinting0104 chemical sciencesApple fruit juice and ciderFruit and Vegetable JuicesBiochemistryFruitMalusProteomeUniProtCombinatorial peptide ligand library0210 nano-technologyApple Fruit JuiceELECTROPHORESIS
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